3DKE
Polar and non-polar cavities in phage T4 lysozyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796,0.9797,0.9742,0.9807 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.226, 60.226, 96.677 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.130 - 1.250 |
| R-factor | 0.15626 |
| Rwork | 0.155 |
| R-free | 0.17281 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.200 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.115 | 0.214 |
| Number of reflections | 56299 | |
| <I/σ(I)> | 23.2 | 13.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | 2.0-2.2 M NaH2PO4 and K2HPO4, pH 6.9, 5mM BME, 5mM oxidized BME, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
