3DJG
Catalytic cycle of human glutathione reductase near 1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-06 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.532, 63.362, 84.619 |
| Unit cell angles | 90.00, 121.54, 90.00 |
Refinement procedure
| Resolution | 20.060 - 1.800 |
| R-factor | 0.145 |
| Rwork | 0.141 |
| R-free | 0.18600 |
| Starting model (for MR) | 3grs |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.662 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.060 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 46862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 3% ammonium sulfate, 0.1 M potassium phosphate and 0.1% beta-octyl glucoside, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00 |
