3DJ9
Crystal Structure of an isolated, unglycosylated antibody CH2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.144, 40.678, 39.131 |
| Unit cell angles | 90.00, 106.74, 90.00 |
Refinement procedure
| Resolution | 26.361 - 1.750 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hzh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (PHENIX.REFINE) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 | 0.116 |
| Number of reflections | 11044 | |
| <I/σ(I)> | 29.9 | 5.4 |
| Completeness [%] | 91.5 | 57.8 |
| Redundancy | 3.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | 30% PEG 1500, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
