3DJ4

Crystal Structure of GlmU from Mycobacterium tuberculosis in complex with URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	H 3
Unit cell lengths	78.600, 78.600, 278.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.030 - 2.380
R-factor	0.197
Rwork	0.194
R-free	0.25700
RMSD bond length	0.027
RMSD bond angle	2.544
Data scaling software	XSCALE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.440
High resolution limit [Å]	2.380	2.380
Rmerge	0.097	0.443
Number of reflections	25334	1553
<I/σ(I)>	19.84	4.3
Completeness [%]	98.3	82.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	8% PEG 8000, 150mM NaCl, 2mM MnCl2, 5% Glycerol, 1,3-butanediol, AMPPNP, MgCl2, DTT, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K