3DIN

Crystal structure of the protein-translocation complex formed by the SecY channel and the SecA ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	101.616, 156.003, 358.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 4.500
R-factor	0.279
Rwork	0.279
R-free	0.30300
Structure solution method	SAD, MOLECULAR REPLACEMENT
Starting model (for MR)	1tf2
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.770
High resolution limit [Å]	4.500	4.500
Number of reflections	34733
<I/σ(I)>	16.1	2
Completeness [%]	97.7	89.7
Redundancy	11.6	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	298	20% PEG 3350, 200mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, temperature 298K
1		7.5	298	20% PEG 3350, 200mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, temperature 298K
1		7.5	298	20% PEG 3350, 200mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, temperature 298K
1		7.5	298	20% PEG 3350, 200mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, temperature 298K