3DHM
Beta 2 microglobulin mutant D59P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.186, 29.189, 55.323 |
| Unit cell angles | 90.00, 121.45, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.800 |
| R-factor | 0.19133 |
| Rwork | 0.190 |
| R-free | 0.22237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z9t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.113 | 0.421 |
| Number of reflections | 9537 | |
| <I/σ(I)> | 12.2 | 2.4 |
| Completeness [%] | 94.9 | 76 |
| Redundancy | 5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Na acetate, PEG 4000, Ammonium acetate, glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
