3DH3
Crystal Structure of RluF in complex with a 22 nucleotide RNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2007-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.552, 84.128, 91.354 |
| Unit cell angles | 90.00, 94.87, 90.00 |
Refinement procedure
| Resolution | 49.510 - 3.000 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.27899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2GML |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.854 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.121 | 0.057 | 0.480 |
| Number of reflections | 27018 | ||
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 99.2 | 98.9 | 95.1 |
| Redundancy | 3.3 | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 200 mM Ammonium acetate, 25% PEG-3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium acetate | ||
| 2 | 1 | 1 | PEG-3350 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Ammonium acetate | ||
| 5 | 1 | 2 | PEG-3350 | ||
| 6 | 1 | 2 | H2O |
