3DGH
Crystal Structure of Drosophila Thioredoxin Reductase, C-terminal 8-residue truncation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 135.755, 135.755, 132.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.770 - 1.745 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.810 |
| High resolution limit [Å] | 1.745 | 1.745 |
| Rmerge | 0.085 | 0.540 |
| Number of reflections | 125246 | |
| <I/σ(I)> | 11.6 | 4.02 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 0.1 M Succinate, 1.0 M Li2SO4, 0.6 M (NH4)2SO4, pH 5.5, vapor diffusion, hanging drop, temperature 303K |
