3DFG
Crystal Structure of RecX: A Potent Inhibitor Protein of RecA from Xanthomonas campestris
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97997, 0.96472 |
| Spacegroup name | P 43 |
| Unit cell lengths | 45.100, 45.100, 93.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.350 - 1.500 |
| Rwork | 0.198 |
| R-free | 0.22730 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | |
| Number of reflections | 55383 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
