3DEP
Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9310 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 66.473, 66.473, 219.703 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.700 |
| R-factor | 0.252 |
| Rwork | 0.249 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3DEO |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.452 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.103 | 0.451 |
| Total number of observations | 8065 | |
| Number of reflections | 8435 | |
| <I/σ(I)> | 3.6 | 3.6 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | VAPOR DIFFUSION, HANGING DROP, temperature 293K |
