3DEN
Structure of E. coli DHDPS mutant Y107W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.196, 121.196, 110.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.220 - 2.200 |
| R-factor | 0.18586 |
| Rwork | 0.183 |
| R-free | 0.24098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.826 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.670 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.432 |
| Number of reflections | 63201 | |
| <I/σ(I)> | 8.2 | 2.8 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.99 | 3.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 284 | 1.8M K2HPO4, 6% (w/v) N-octyl-beta-R-glucopyranoside , pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 284K |
