3DDJ
Crystal structure of a cbs domain-containing protein in complex with amp (sso3205) from sulfolobus solfataricus at 1.80 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.560, 48.090, 83.280 |
| Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
| Resolution | 29.617 - 1.800 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.617 | 1.860 | |
| High resolution limit [Å] | 1.800 | 3.870 | 1.800 |
| Rmerge | 0.047 | 0.023 | 0.529 |
| Number of reflections | 28983 | 5708 | 4948 |
| <I/σ(I)> | 17.67 | 45.3 | 2.5 |
| Completeness [%] | 99.1 | 99.7 | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.76 | 293 | 23.0% polyethylene glycol 600, 0.1M phosphate-citrate pH 3.76, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
