3DD7
Structure of DocH66Y in complex with the C-terminal domain of Phd
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9189, 0.9116 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.944, 38.201, 63.734 |
| Unit cell angles | 90.00, 99.26, 90.00 |
Refinement procedure
| Resolution | 11.000 - 1.700 |
| R-factor | 0.18317 |
| Rwork | 0.182 |
| R-free | 0.19849 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.237 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.950 |
| Number of reflections | 29371 | |
| <I/σ(I)> | 9 | 2 |
| Redundancy | 7.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 35% PEG4000, 0.2M ammonium acetate, 0.1M acetate, pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
