3DD4

Structural Basis of KChIP4a Modulation of Kv4.3 Slow Inactivation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 41
Unit cell lengths	96.300, 96.300, 71.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.2392
Rwork	0.239
R-free	0.29880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	KChIP1 (PDB code: 2NZ0)
RMSD bond length	0.008
RMSD bond angle	1.210
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.058	0.567
Number of reflections	6650
<I/σ(I)>	37.9	4.1
Completeness [%]	99.6	100
Redundancy	8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6-1.8M Ammonium Sulfate, 4%(v/v) iso-Propanol, 0.1M DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K