3DCP
Crystal structure of the putative histidinol phosphatase hisK from Listeria monocytogenes. Northeast Structural Genomics Consortium target LmR141.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 64 |
| Unit cell lengths | 191.278, 191.278, 48.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.560 - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | |
| Rmerge | 0.106 | 0.272 |
| Number of reflections | 114574 | |
| <I/σ(I)> | 23.6 | 4.4 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 15% PEG 8000, 0.17 M sodium acetate, 0.01 M L-cysteine, 0.1 M MES pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
