3DC7

Crystal structure of the protein Q88SR8 from Lactobacillus plantarum. Northeast Structural Genomics consortium target LpR109.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9791, 0.9679, 0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	48.610, 101.440, 125.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 2.120
R-factor	0.18598
Rwork	0.184
R-free	0.22431
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.120	2.270
Rmerge	0.078	0.229
Number of reflections	54518
<I/σ(I)>	20.4	7.5
Completeness [%]	99.1	97.5
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M MgSo4, 0.1M Bis-Tris, 16% PEG8K, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K