3DBJ
Allophycocyanin from Thermosynechococcus vulcanus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 3 |
Unit cell lengths | 102.597, 102.597, 128.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
Rwork | 0.212 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1all |
RMSD bond length | 0.008 |
RMSD bond angle | 1.410 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.091 | 0.248 |
Number of reflections | 30684 | |
<I/σ(I)> | 9.1 | 3.7 |
Completeness [%] | 93.0 | 88.8 |
Redundancy | 2.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 6% PEG 4000, 400mM MgSO4, 20mM Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |