3DBA
Crystal structure of the cGMP-bound GAF a domain from the photoreceptor phosphodiesterase 6C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-25 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.598, 67.598, 198.533 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.850 - 2.570 |
| R-factor | 0.22245 |
| Rwork | 0.221 |
| R-free | 0.25705 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Number of reflections | 17389 | |
| <I/σ(I)> | 51.8 | 4.8 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 10.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 295 | 1.5 M ammonium sulfate, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
