3DAR

Crystal structure of D2 domain from human FGFR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	P 2 21 21
Unit cell lengths	41.870, 78.240, 85.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.917 - 2.200
R-factor	0.2188
Rwork	0.212
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PBD Entry 1E0O chain B residues 149-249
RMSD bond length	0.008
RMSD bond angle	1.149
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.900	33.920	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge	0.080	0.031	0.623
Total number of observations		3509	15291
Number of reflections	14667
<I/σ(I)>	6.7	16.3	1.2
Completeness [%]	98.8	97.5	98.1
Redundancy	7.3	6.7	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 M sodium acetate, 0.1 M Tris-HCl, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K, pH 8.5