3D89

Crystal Structure of a Soluble Rieske Ferredoxin from Mus musculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.96419
Spacegroup name	P 43 21 2
Unit cell lengths	52.413, 52.413, 108.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.750 - 2.071
R-factor	0.2
Rwork	0.199
R-free	0.22800
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.848
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.070	4.460	2.070
Rmerge	0.057	0.038	0.621
Number of reflections	9806
<I/σ(I)>	18.354		1.845
Completeness [%]	99.4	98.7	95.5
Redundancy	12.7	12.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Protein Solution (~15 mg/ml native protein, 0.025 M NaCl, 0.005 M MOPS pH 7.0) mixed in a 1:1 ratio with Well Solution ( 25% MEPEG 5K, 0.12 M Trisodium citrate, 0.10 M MES ph 6.0) Cryoprotected with 15% ethylene glycol; Mercury derivative crystal soaked overnight in in mother liquor containing 0.002 M thimersol, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	6	293	Protein Solution (~15 mg/ml native protein, 0.025 M NaCl, 0.005 M MOPS pH 7.0) mixed in a 1:1 ratio with Well Solution ( 25% MEPEG 5K, 0.12 M Trisodium citrate, 0.10 M MES ph 6.0) Cryoprotected with 15% ethylene glycol; Mercury derivative crystal soaked overnight in in mother liquor containing 0.002 M thimersol, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP