3D7M

Crystal Structure of the G Protein Fast-Exchange Double Mutant I56C/Q333C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	79.659, 79.659, 114.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
Rwork	0.249
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gfi
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge		0.301
Number of reflections	8576
<I/σ(I)>	12.6	1.88
Completeness [%]	96.8	80.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	16% PEG 3350, 0.24 M Li(CH3COO), 0.1 M Bis-Tris, 0.01 M SrCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K