3D7C
Crystal structure of the bromodomain of human GCN5, the general control of amino-acid synthesis protein 5-like 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-31 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.428, 72.862, 75.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.410 - 2.060 |
R-factor | 0.18272 |
Rwork | 0.179 |
R-free | 0.25654 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oss |
RMSD bond length | 0.016 |
RMSD bond angle | 1.503 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.2) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.410 | 2.170 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.129 | 0.736 |
Number of reflections | 16003 | |
<I/σ(I)> | 9.7 | 2 |
Completeness [%] | 98.9 | 99.2 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |