3D79
Crystal structure of hypothetical protein PH0734.1 from hyperthermophilic archaea Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.921, 52.921, 133.313 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.730 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 3.720 | 1.730 |
| Rmerge | 0.065 | 0.055 | 0.221 |
| Number of reflections | 23373 | ||
| <I/σ(I)> | 21.3 | ||
| Completeness [%] | 99.8 | 98.7 | 100 |
| Redundancy | 18.8 | 18.4 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
