3D6X
Crystal structure of Campylobacter jejuni FabZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.589, 93.403, 126.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.160 - 2.590 |
| R-factor | 0.21823 |
| Rwork | 0.216 |
| R-free | 0.25361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2glm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.582 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.072 | 0.264 |
| Number of reflections | 29418 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 88.2 | 60.5 |
| Redundancy | 3.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 288 | 14% Polyethylene glycol monomethylether 550, 0.2M NaCl, 0.1M BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
