3D6O
The RNase A- 5'-Deoxy-5'-N-(ethyl isonipecotatyl)uridine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.384, 32.876, 72.621 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.580 |
| R-factor | 0.18793 |
| Rwork | 0.186 |
| R-free | 0.22678 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.3) |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.057 | |
| Number of reflections | 31074 | |
| <I/σ(I)> | 16.5 | 3.8 |
| Completeness [%] | 94.9 | 96.6 |
| Redundancy | 7.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 4000, Sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
