3D57
TR Variant D355R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-09-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.557, 92.963, 58.808 |
| Unit cell angles | 90.00, 110.02, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nax |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.370 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.210 |
| Number of reflections | 28545 | |
| <I/σ(I)> | 11 | 3 |
| Completeness [%] | 91.0 | 90.2 |
| Redundancy | 2.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 25% ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
