3D4J
Crystal structure of Human mevalonate diphosphate decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.960, 53.190, 97.790 |
| Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
| Resolution | 29.450 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fi4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.450 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 33621 | |
| <I/σ(I)> | 18.4 | 2.9 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 292 | MES buffer, PEG5KMME, Ammonium Sulfate, NaCl, pH 6.5, VAPOR DIFFUSION, temperature 292K |
