3D4G

ZP-N domain of mammalian sperm receptor ZP3 (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-07
Detector	MAR CCD 165 mm
Wavelength(s)	0.95350
Spacegroup name	P 1
Unit cell lengths	91.050, 91.960, 140.480
Unit cell angles	89.99, 90.18, 89.98

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.2017
Rwork	0.201
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d4c
RMSD bond length	0.007
RMSD bond angle	0.927
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.3.0038)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.350
High resolution limit [Å]	2.300	2.300
Number of reflections	193240
<I/σ(I)>	11	1.6
Completeness [%]	95.4	95.7
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sample: 15mg/ml protein in 0.05M sodium chloride, 0.01M Tris-HCL, PH7.2, 0.001M maltose. Reservoir: 10% PEG6000, 0.2M calcium chloride, 0.1M Tris-HCL, PH7.0. Sample to reservoir ratio in drop: 1:1, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K