3D4G
ZP-N domain of mammalian sperm receptor ZP3 (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.95350 |
Spacegroup name | P 1 |
Unit cell lengths | 91.050, 91.960, 140.480 |
Unit cell angles | 89.99, 90.18, 89.98 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.2017 |
Rwork | 0.201 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d4c |
RMSD bond length | 0.007 |
RMSD bond angle | 0.927 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0038) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 193240 | |
<I/σ(I)> | 11 | 1.6 |
Completeness [%] | 95.4 | 95.7 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sample: 15mg/ml protein in 0.05M sodium chloride, 0.01M Tris-HCL, PH7.2, 0.001M maltose. Reservoir: 10% PEG6000, 0.2M calcium chloride, 0.1M Tris-HCL, PH7.0. Sample to reservoir ratio in drop: 1:1, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |