3D4D
Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-02-06 |
Detector | BRUKER APEX II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.022, 81.293, 60.385 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 60.380 - 2.100 |
R-factor | 0.239 |
Rwork | 0.233 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.316 |
Data reduction software | SAINT |
Data scaling software | SAINT (V7.51A) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.380 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.100 |
Number of reflections | 17583 | |
<I/σ(I)> | 38.84 | 15.41 |
Completeness [%] | 99.9 | |
Redundancy | 11.3 | 4.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K |