3D45
Crystal structure of mouse PARN in complex with m7GpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.013, 128.350, 176.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.690 - 3.000 |
| R-factor | 0.30035 |
| Rwork | 0.299 |
| R-free | 0.33371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2A1R 1WHV |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.532 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.690 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.101 | 0.327 |
| Number of reflections | 29592 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 6~10% PEG6000, 100mM MES, pH6.0, 10mM betaine , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
