3D3I

Crystal structural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63

Replaces:  2DS3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97912
Spacegroup name	P 1 21 1
Unit cell lengths	61.687, 138.549, 87.543
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	50.000 - 1.780
Rwork	0.169
R-free	0.20100
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.780
Number of reflections	139299

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	20% PEG 8000, 0.6M magnesium chloride, 100mM Tris-HCl buffer, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K