3D34

Structure of the F-spondin domain of mindin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.600, 68.027, 110.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.800
Rwork	0.189
R-free	0.21500
Data reduction software	DENZO
Data scaling software	d*TREK (9.2SSI)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.730	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.052	0.163
Total number of observations		9255
Number of reflections	35173
<I/σ(I)>	13.5	5.8
Completeness [%]	97.3	93.7
Redundancy	3.08	2.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	0-6% PEG2000, 100mM Tris-HCl, 10mM NiCl2, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K