3D32
Complex of GABA(A) receptor-associated protein (GABARAP) with a synthetic peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.287, 35.415, 58.209 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 27.170 - 1.300 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.041 | 0.320 |
| Number of reflections | 60022 | |
| <I/σ(I)> | 16.3 | 2.7 |
| Completeness [%] | 93.9 | 92.3 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.9 | 285 | 30 % (w/v) PEG3350 800 mM NaCl 50 mM MES, pH 5.9, VAPOR DIFFUSION, temperature 285K |
