3D2N

Crystal structure of MBNL1 tandem zinc finger 1 and 2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2821
Spacegroup name	P 31
Unit cell lengths	49.320, 49.320, 36.026
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.23284
Rwork	0.230
R-free	0.27942
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	5336
<I/σ(I)>		2.7
Completeness [%]	98.7	90.1
Redundancy	2.9	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	277	20% PEG 4000, 0.1M sodium citrate, 20 % isopropanol, pH 7.2, VAPOR DIFFUSION, temperature 277K