3D1P
Atomic resolution structure of uncharacterized protein from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.6525 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 58.343, 37.429, 52.198 |
Unit cell angles | 90.00, 108.86, 90.00 |
Refinement procedure
Resolution | 30.000 - 0.980 |
R-factor | 0.10897 |
Rwork | 0.108 |
R-free | 0.12369 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.723 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.000 |
High resolution limit [Å] | 0.980 | 0.980 |
Rmerge | 0.062 | 0.650 |
Number of reflections | 59810 | |
<I/σ(I)> | 20 | 1.9 |
Completeness [%] | 98.3 | 95.5 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 2.4 M Ammonium sulfate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |