3D1N
Structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone gene promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.298, 112.060, 181.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.910 - 2.510 |
| R-factor | 0.2119 |
| Rwork | 0.212 |
| R-free | 0.26980 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 64438 | |
| <I/σ(I)> | 17.9 | 1.63 |
| Completeness [%] | 91.0 | 52.9 |
| Redundancy | 9.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.1 M K-Na Tartrate Tetrahydrate, 0.05 M MgCl2, 20 % PEG3350, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | K-Na Tartrate Tetrahydrate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | PEG3350 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | K-Na Tartrate Tetrahydrate | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | PEG3350 | ||
| 8 | 1 | 2 | H2O |
