3D0U
Crystal Structure of Lysine Riboswitch Bound to Lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1050 |
Spacegroup name | P 32 |
Unit cell lengths | 119.823, 119.823, 58.744 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.619 - 2.800 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.20900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.087 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.084 | 0.403 |
Number of reflections | 24657 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 95.6 | 61.4 |
Redundancy | 5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 2 M Li2SO4, 5 mM MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Li2SO4 | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 2 | Li2SO4 | ||
4 | 1 | 2 | MgCl2 |