3D0T
Structure of the BNB domain of the Hsp70 cochaperone Bag2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-02-10 |
Detector | NOIR-1 |
Wavelength(s) | 0.96410908, 0.99505962 |
Spacegroup name | H 3 2 |
Unit cell lengths | 104.680, 104.680, 164.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.690 - 2.550 |
Rwork | 0.253 |
R-free | 0.28400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.340 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.059 | 0.242 |
Total number of observations | 6731 | |
Number of reflections | 11398 | |
<I/σ(I)> | 14.5 | 5.7 |
Completeness [%] | 98.8 | 99.9 |
Redundancy | 5.96 | 5.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 35% PEG 400, 0.1M Bis-Tris pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |