3D0P
Insights into RNA/DNA hybrid recognition and processing by RNase H from the crystal structure of a non-specific enzyme-dsDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97850 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.397, 66.660, 76.926 |
| Unit cell angles | 90.00, 122.31, 90.00 |
Refinement procedure
| Resolution | 41.590 - 1.800 |
| R-factor | 0.21539 |
| Rwork | 0.214 |
| R-free | 0.24108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbi using one protein molecule. |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.248 |
| Number of reflections | 37768 | |
| <I/σ(I)> | 12.4 | 12.4 |
| Completeness [%] | 97.3 | 94.7 |
| Redundancy | 7.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M sodium acetate, 8 % (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 2 | sodium acetate | ||
| 4 | 1 | 2 | PEG 4000 |
