3D0O
Crystal structure of Lactate Dehydrogenase from Staphylococcus Aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Collection date | 2008-04-28 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.765, 74.503, 96.306 |
| Unit cell angles | 90.00, 129.07, 90.00 |
Refinement procedure
| Resolution | 29.370 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.524 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.084 | 0.070 | 0.259 |
| Number of reflections | 75432 | ||
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 94.6 | 97.8 | 67.1 |
| Redundancy | 2.8 | 3.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
