3D07
Human p53 core domain with hot spot mutation R249S (III)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.224, 68.477, 57.832 |
Unit cell angles | 90.00, 101.34, 90.00 |
Refinement procedure
Resolution | 34.240 - 2.200 |
R-factor | 0.16778 |
Rwork | 0.163 |
R-free | 0.25627 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tsr |
RMSD bond length | 0.019 |
RMSD bond angle | 1.912 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (- CCP4) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 19630 | |
<I/σ(I)> | 13.1 | 4.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.65 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.2M Sodium Acetate, 20% PEG 3350, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |