3D06
Human p53 core domain with hot spot mutation R249S (I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93930 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 44.157, 44.157, 329.369 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.340 - 1.200 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tsr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (- CCP4) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.340 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 59415 | |
| <I/σ(I)> | 28.1 | 2.4 |
| Completeness [%] | 96.1 | 75.4 |
| Redundancy | 4.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.15M Sodium acetate, 20% PEG 3350, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
