3D00
Crystal structure of a tungsten formylmethanofuran dehydrogenase subunit e (fmde)-like protein (syn_00638) from syntrophus aciditrophicus at 1.90 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-03 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97817 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 54.356, 54.356, 136.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.386 - 1.900 |
R-factor | 0.235 |
Rwork | 0.233 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.604 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.386 | 29.386 | 1.950 |
High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
Rmerge | 0.078 | 0.011 | 0.011 |
Total number of observations | 1244 | 8626 | |
Number of reflections | 16955 | ||
<I/σ(I)> | 6.8 | 16.4 | 0.7 |
Completeness [%] | 99.9 | 95.9 | 99.9 |
Redundancy | 7 | 5.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.01M NiCl2, 20.0% PEG MME 2000, 0.1M Tris-HCl pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |