3CZM
T. Gondii bradyzoite-specific LDH (LDH2) in complex with NAD and OXQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Detector technology | CCD |
| Collection date | 2008-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97946 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 140.949, 140.949, 75.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.350 - 2.300 |
| R-factor | 0.16859 |
| Rwork | 0.166 |
| R-free | 0.22253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sow |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.350 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 35999 |
| Completeness [%] | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 14% PEG 5K, .6M Magnesium Acetate, .1M Sodium Acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
