3CZ2

Dimeric crystal structure of a pheromone binding protein from Apis mellifera at pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.886
Spacegroup name	P 21 21 21
Unit cell lengths	35.862, 75.513, 84.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.500
R-factor	0.22578
Rwork	0.220
R-free	0.27838
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3czy
RMSD bond length	0.012
RMSD bond angle	1.213
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.115	0.313
Number of reflections	8426
<I/σ(I)>	14.6	5.1
Completeness [%]	99.9	100
Redundancy	6.2	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2M Magnesium chloride, 0.1M Tris, 17% PEG8000, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K