3CXQ
Crystal structure of human glucosamine 6-phosphate N-acetyltransferase 1 bound to GlcN6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-03 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.136, 54.136, 138.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2huz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.139 |
| Data scaling software | LSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.348 | 54.350 | 2.460 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Number of reflections | 8884 | ||
| <I/σ(I)> | 9.2 | 16.4 | 1.3 |
| Completeness [%] | 90.4 | ||
| Redundancy | 6.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M Na formate, 25% w/v PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
