3CXL
Crystal structure of human chimerin 1 (CHN1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 62 |
| Unit cell lengths | 152.187, 152.187, 69.529 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.513 - 2.600 |
| R-factor | 0.267 |
| Rwork | 0.266 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1XA6 2osa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.053 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.082 | 0.038 | 0.970 |
| Number of reflections | 29085 | ||
| <I/σ(I)> | 15 | ||
| Completeness [%] | 99.7 | 97.4 | 97.3 |
| Redundancy | 10.8 | 10 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | 1.4M Ammonium sulfate, 0.1M Glycine, 0.01M Magnesium chloride, 5% MPD, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
