3CXH
Structure of yeast complex III with isoform-2 cytochrome c bound and definition of a minimal core interface for electron transfer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.540, 162.970, 194.230 |
| Unit cell angles | 90.00, 104.39, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1KB9 and 1YEA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 289871 | |
| <I/σ(I)> | 12.7 | 2.8 |
| Completeness [%] | 92.7 | 77.5 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch (Paraffin oil) | 7.5 | 277 | 1M Sucrose, 10% DMSO, 20mM Tris pH 7.5, 80mM NaCl, 0.05 % UM, 1 M stigmatellin, 5% PEG 4000, Microbatch (Paraffin oil), temperature 277K |
