3CWZ

Structure of RAB6(GTP)-R6IP1 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.978
Spacegroup name	P 61 2 2
Unit cell lengths	100.240, 100.240, 299.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.970 - 3.200
R-factor	0.246
Rwork	0.246
R-free	0.30200
Structure solution method	SAD
RMSD bond length	2.823
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.060
Number of reflections	18629
<I/σ(I)>	13.3
Completeness [%]	99.6
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M HEPES, 2% PEG 4000, 3% MPD, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M HEPES, 2% PEG 4000, 3% MPD, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K