3CWN
Escherichia coli transaldolase b mutant f178y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.864, 86.173, 131.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.000 - 1.400 |
| R-factor | 0.15009 |
| Rwork | 0.149 |
| R-free | 0.17257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.212 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.037 | 0.150 |
| Number of reflections | 135268 | |
| <I/σ(I)> | 21.4 | 5.7 |
| Completeness [%] | 92.0 | 62 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 18 % PEG 3350, 0.2 M ammonium sulfate, 25 mM glycylglycine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
